Uji Akivitas Imunomodulator Ekstrak Buah Petai (Parkia speciosa Hassk.) terhadap Titer Imunoglobulin (IgG) pada Mencit Balb/c yang Diinduksi dengan SDMD

Yithro Serang, Ferika Indrasari

Abstract


Immunomodulators are substances or drugs that can modulate the function and activity of the immune system. Most of the medicinal plants that have been studied prove the presence of immunostimulators, while immunosuppressors are still rare. Petai plants can function as antioxidants. There is a close relationship between antioxidants and the immune system giving antioxidants can stimulate and protect the cellular immune system. The purpose of this study was to determine the effect of administration and effective dose of petai fruit (Parkia speciosa Hassk) ethanol extract on increasing IgG titer in Balb / c mice induced by SDMD.

This research method was started with Petai extraction and then tested for chemical content. Experimental animals consisted of 25 mice divided into 5 treatment groups, each group consisting of 5 mice. All groups were immunized with 1% SDMD as much as 0.2 ml / 20 g BB on day 8 (after being acclimatized for 7 days) intravenously. Test preparation is given orally for 7 days. On day 14, blood collection from retro orbital plexus was performed to collect serum and examined immunoglobulin G titers with indirect ELISA method.

The benefit to be obtained from this study is to prove the ethanol extract of petai fruit (Parkia speciosa Hassk) can increase the immune system against foreign compounds with an increase in IgG titer in Balb / c mice induced by HRD. The results of Petai Fruit Extract Dosage 4.125 mg / KgBB, 8.25 mg / Kg BB and a dose of 16.5 mg / Kg BB give an effect on increasing IgG titers. The dose of 16.5 mg is better than stimouno in increasing IgG levels of mice induced by HRD.

Keywords Petai (Parkia speciosa Hassk), IgG, SDMD,ELISA


Full Text:

References


. Achyat, S.R., Sadikin, M., Jusman, S.W.A.J., Rusdi. 2008. Pengaruh Pemberian Minyak Buah Merah (Pandanus conoideus Lam) terhadap Imunitas Humoral Tikus (Ratus norvegicus L.) Galur Wistar Melalui Pengamatan Titer Antibodi Anti-SDMD. ISSN 1412-2855. Vol. 6.

. Anonim, 2007. http://www.plantamor.com/index.php?plant=1377, diunduh pada tanggal 20 Maret 2012.

. Baratawidjaja KG. 2009. Imunologi Dasar. Edisi VIII. Jakarta: Balai Penerbit Fakultas Kedokteran Universitas Indonesia. hlm 27-55, 140-176, 412-428, 515-528, 546-554, 560-561, 577-578.

. Bellanti JA. (1993). Imunologi III. Penerjemah A.A. Wahab. Yogyakarta. Gajah Mada

. Block, K.I. and M.N. Mead. 2003. Immune system effects of Echinacea, Ginseng and Astragalus: A review Integrative cancer therapies. 2(3): 247 – 267.

. Casc CL, Funke BR, Tortora GJ. 2001. Microbiology an introduction. San Francisco: 7th Ed. Addison Wesley Longman. Inc.

. Kresno SB. 2001. Imunologi : Diagnosis dan Prosedur Laboratorium. Ed ke-4. Jakarta: Balai Penerbit Fakultas Kedokteran Universitas Indonesia.

. Laily N., 2010, Pengembangan Pangan Darurat Berigizi dan Berkhasiat Peningkat Daya Tahan Tubuh, Laporan Akhir Kegiatan Program Insentif, BPPT.

. Roitt, I., J. Brostoff, and D. Male, 2001, Immunology, 10th ed., Gower Medical Publ., London, 1-10.

. Sheehan C. 1997. Clinical Imunology Priciple And Laboratory Diagnosis. 2nd Ed. Lippincott Company. Philladelphia. New York.

. Shukla dkk. (2009). Immunomodulatory activities of the ethanolic extract of Caesalpinia bonducella seeds. Journal of Ethnopharmacology 125. Page 252-256.

. Smith, Mangkoewidjaja. 1988. Pemeliharaan Pembiakan Hewan Percobaan di Daerah Tropis. Jakarta: UI Press.

. Tjay TH, Rahardja K. 2002. Obat-Obat Penting. Jakarta: PT.Elex Media Komputindo.

. Voigt, Rudolf. 1995. Buku Pelajaran Teknologi Farmasi. Ed ke- 5. Yogyakarta: Universitas Gadjah Mada Press.




DOI: http://dx.doi.org/10.30591/pjif.v8i1.1306

Refbacks

  • There are currently no refbacks.


Copyright (c) 2019 Parapemikir : Jurnal Ilmiah Farmasi

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License

 My Counter

.

 

 

===============================================================

 

 

 

Indexed By: