PENENTUAN NILAI SPF ( SUN PROTECTION FACTOR) EKSTRAK DAN FRAKSI DAUN KECOMBRANG (ETLINGERA ELATIOR) SECARA IN VITRO MENGGUNAKAN METODE SPEKTROFOTOMETRI

Oktariani Pramiastuti

doi:10.30591/pjif.v8i1.1281

PENENTUAN NILAI SPF ( SUN PROTECTION FACTOR) EKSTRAK DAN FRAKSI DAUN KECOMBRANG (ETLINGERA ELATIOR) SECARA IN VITRO MENGGUNAKAN METODE SPEKTROFOTOMETRI

Oktariani Pramiastuti

Abstract

Sunlight is needed by all living things to their survival. One of the benefits of sunlight is as sources of energy and making healthy skin and bones. Kecombrang leaf contains phenolic compound which is able to give protection for UV light and have potentially sunscreens. The research aimed to determine of SPF of water extract, n-hexane fraction, and ethyl acetate fraction of kecombrang leaf. The research was in vitro by using spectrophotometry UV-Vis method. The absorbance of extract and fraction of kecombrang leaf was measured at a UV-B light wave length of 290-320 nm. Determination of SPF was based on Mansur equation. Phytochemical test in TLC of water extract, n-hexane fraction, and ethyl acetate fraction was positive for phenol in each. The result showed that water extract of kecombrang leaf had the highest SPF at concentration of 300 ppm numbered 7.30±0.62 with level of extra sunscreens ability; n-hexane fraction of kecombrang leaf had the highest protection activity in a SPF of 17.57±2.49 with level of ultra-sunscreens ability in a concentration of 300 ppm; while ethyl acetate fraction had the highest SPF namely 2.65±0.12 with the minimum protection. The data analysis stated that there was a significant difference between water extract of kecombrang leaf and fractions of n-hexane and ethyl acetate. To sum up, n-hexane fraction of kecombrang leaf had the highest SPF so that it had potentially as sunscreens for UV-B light protection

Keywords: Kecombrang Leaf, SPF, Water Extract, n-hexane fraction, ethyl acetate fraction

Full Text:

References

Ismail, I., Handayany. W.G., Wahyuni, D., Juliandri. 2014. Formulasi dan Penentuan Nilai SPF (Sun Proetecting Factor) Sediaan Krim Tabir Surya Ekstrak Etanol Daun Kemangi ( Ocimum sanctum L). JF FIK UINAM

Shovyana, H.H., A. Karim Zulkarnain. 2013. Physical Stability and Activity of Cream W/O Etanolic Fruit Extract of Mahkota Dewa (Phaleria macrocarpha (Scheff.) Boerl) as A a Sunscreen. Traditional Medicine Journal 18(2), Fakultas Farmasi UGM. Yogyakarta.

Prastiyo.A.B., Pramiastuti.O dan Zen. D.A. 2018. Penentuan Kadar Total Fenolik dan Uji Aktivitas Antioksidan Ekstrak Etanol Daun Kecombrang Menggunakan Metode DPPH. Skripsi. STIKes Bhamada Slawi.

Sa’adah, L. 2010. Isolasi dsan Identifikasi Senyawa Tanin dari Daun Belimbing Wuluh (Averrhoa bilimbi L). Jurusan Kimia UIN Maulana Malik Ibrahim Malang

Damogalad, V., Hosea Jaya Edy dan Hamidah Sri Supriadi. 2013. Formulasi Krim Tabir Surya Ekstrak Kulit Nanas (Ananas comosus L Merr) dan Uji In Vitro Nilai Sun Protecting Factor ( SPF). Pharmacon Jurnal Ilmiah Farmasi UNSRAT Vol 2. No.2. Manado. Program Studi Farmasi FMIPA UNSRAT.

Pratama, A.W., dan Zulkarnain K. 2015. Uji SPF in Vitro dan Sifat Fisik Beberapa Produk Tabir Surya yang Beredar di Pasaran. Majalah Farmaseutik, Vol 11 No.1. Fakultas Farmasi UGM Yogyakarta.

Mansur JS, et al. 1986. Determination of Sun Protection Factor for Spectrophotometry. An Bras Deramtol.

Yulianti. E., Adelsa. A dan Putri. A. 2015. Penentuan Nilai SPF (Sun Protecting Factor) Ekstrak Etanol 70 % Temu Mangga (Curcuma mangga) dan Krim ekstrak etanol 70 % Temu Mangga (Curcuma mangga) Secara In Vitro Menggunakan Metode Spektrofotometri. Majalah Kesehatan. Vol 2, No.1. FKUB.

Suyatno, Hidajati, Syarief N., Sri Hidayati, Rinaningsih & Wakhida Hidayatin Nur. 2007. Uji In Vitro Aktivitas Tabir Surya Senyawa Turunan Sinamat Hasil Isolasi dari Rimpang Kencur (Kaempferia galangal L.).

Harborne, J.B. 1987. Metode Fitokimia: Penuntun Cara Modern Menganalisis Tumbuhan. Bandung: Penerbit ITB.

Wihelmina C.E. 2011. Pembuatan dan Penentuan Nilai SPF Nanoemulasi Tabir Surya Menggunakan Minyak Kencur (Keampferia galanga L.) Sebagai fase MinyakSkripsi.

DOI: https://doi.org/10.30591/pjif.v8i1.1281